DNA Microarray Analysis of Complex Biologic Processes

SCIENCE WATCH DNA Microarray Analysis of Complex Biologic Processes

DNA microarrays, or gene chips, allow surveys of gene expression, (i.e., mRNA expression) in a highly parallel and comprehensive manner. The pattern of gene expression produced, known as the expression profile, depicts the subset of gene transcripts expressed in a cell or tissue. At its most fundamental level, the expression profile can address qualitatively which genes are expressed in disease...

Real-time DNA microarray analysis

We present a quantification method for affinity-based DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e. real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation in the capturing spots, washing artifacts, microarray spot-to-spot variations, and other signal amplitude-aff...

Predicting CpG Islands and DNA Methlation in the Cow Genome Using DNA Microarray Meta-Analysis and Genome Wide Scanning

DNA methylation is a type of epigenetic changes that directly affects DNA. In mammals, DNA methylation is essential for fetal development and stem cell differentiation and this phenomenon essentially occurs within the CpG islands. In this study, two methods were used to study the DNA methylation profile of cow genome. In the first method, the DNA methylation profile of the differentially expres...

A Review of DNA Microarray Data Analysis

Design and Analysis of DNA Microarray Investigations

Microarray experiments have become standard methodology for measuring the expression levels of many genes across a range of cell lines, tissues or other organic material. The decidedly non-standard aspect of such experiments is the subsequent data analysis: the small number of noisy microarrays relative to the large number of genes presents a challenge to biologists using this technology. This ...